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KMID : 0644320060110010119
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Journal of Korean Oriental Oncology
2006 Volume.11 No. 1 p.119 ~ p.134
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Anti-angiogenic Effects of Shiquandabutang
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Cui xun
Kang Hee
Shim Bum-Sang
Kim Sung-Hoon
Choi Seung-Hun
Ahn Kyoo-Suk
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Abstract
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Shiquandabutang is very famous prescription for tonifying vital energy. We examined the anti-metatstastic effect of Shiquandabutang with in vitro invasion assay model. We performed the following experiments and the results are listed below:Cell viability assay was carried to determine the dose of Shiquandabutang. At lower dose under 200 ug/ml (89.6%) viability was very high. But, viability downed as dose grows. At the dose of 600 ug/ml (54.2%) viability was almost half of that of control. And at high dose of 1000 ug/ml (15.8%) viability was very pure. In BrdU incorporation assay, Shiquandabutang treated groups showed the decreased DNA synthesis rate compared with control group.(200 ug/ml (64.4%), 400 ug/ml (7.3%)) The results of gelatinase assay showed that Shiquandabutang decreases the gelatinolytic activity of MMP-9. We examined tube formation assay and the result was that Shiquandabutang ihhibits the tube formation at the dose of 200 ug/ml and 400 ug/ml. We examined rat aortic ring assay and the result was that Shiquandabutang ihhibits the angiogenesis of the rat aortic ring at the dose of 400 ug/ml. From our research, part of the mechanism underlying anti-metastastic effect of Shiquandabutang was proven in vitro. Moreover, we knew that Shiquandabutang is more effectively inhibits the angiogenesis at high dose.
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KEYWORD
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Shiquandabutang, angiogenesis, BCE
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